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CD274 (PD-L1) (C-Term) peptide (Biotin)

PD-L1 Reactivité: Humain Hôte: Synthetic BP, Imm
N° du produit ABIN5539420

Aperçu rapide pour CD274 (PD-L1) (C-Term) peptide (Biotin) (ABIN5539420)

Antigène

PD-L1 (CD274 (PD-L1))

Origine

Humain

Source

  • 6
Synthetic

Application

Blocking Peptide (BP), Immunogen (Imm)
  • Protein Region

    C-Term

    Purification/Conjugué

    This PD-L1 peptide is labeled with Biotin.

    Séquence

    CKKQSDTHLE ET.

    Homologie

    Human
  • Indications d'application

    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 40 kDa band observed in Human Heart lysates (calculated MW of 33.3 kDa according to NP_054862.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.

    Buffer

    100µg of Lyophilised peptide, formulation De-ionized (DI) water.

    Stock

    -20 °C

    Stockage commentaire

    Shipped at ambient temperature, store at -20°C.
  • Antigène

    PD-L1 (CD274 (PD-L1))

    ID gène

    29126

    NCBI Accession

    NP_054862, NP_001254635
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